Basic Chromatography and Separation Technology in Analytical Labs

Start Date End Date Venue Fees (US $)
03 May 2026 Al-Khobar, KSA $ 4,500 Register
30 Aug 2026 Kuala Lumpur, Malaysia $ 4,500 Register
25 Oct 2026 Dubai, UAE $ 3,900 Register

Basic Chromatography and Separation Technology in Analytical Labs

Introduction

The purpose of this course is to provide training in the fundamental techniques of both gas chromatographies (GC) and liquid chromatography (HPLC). The course covers major components of a chromatography; operating principles; application; calibration methods; set-up procedures; preventative maintenance; troubleshooting methods; quantitative methods and failure modes for each, along with practical examples. The course also discusses other aspects such as optimization of column lengths, flows, temperatures, and pressure with the necessary theoretical information in each part. The aim of this course is to enrich and advance the skills and knowledge of participants and to teach them

Objectives

    • To impart participants with the fundamental techniques and knowledge of chromatography.
    • To familiarize participants with the tools and techniques used in chromatography analyses, cause and effect diagrams, calibration, and standard analysis methods.
    • To provide the skills, knowledge, and understanding of principles of chromatography analysis application.
    • To use routine maintenance.
    • To impart participants the operation technique.
    • To train participants to know how to change and install all chromatography components.
    • To be familiar with the latest analytical methods.

Training Methodology

This is an interactive course. There will be open question and answer sessions, regular group exercises and activities, videos, case studies, and presentations on best practice. Participants will have the opportunity to share with the facilitator and other participants on what works well and not so well for them, as well as work on issues from their own organizations. The online course is conducted online using MS-Teams/ClickMeeting.

Who Should Attend?

The course is designed for chemists, lab technicians, chemical engineers, instrument engineers and lab supervisors/managers.

Course Outline

  • Fundamental chromatography technique
  • Chromatographic theory parameters
  • Chromatography retention process
  • Separation methods
  • Application of chromatography
  • Solid phase extraction method
  • Derivatization method
  • Standard operation method
  • Successful and safe operating procedure
  • Gas chromatography technique
  • Carrier gas and pressure regulator system
  • Split/splitless inlet system
  • Cool on-column inlet and programmed temperature vaporization inlet
  • Column configuration
  • Detector configuration
  • Maintenance and installation procedure for inject system, column and detectors
  • Liquid chromatography technique
  • Mobile phase solvent
  • Pumping system
  • Column and detector types
  • Operation method of HPLC
  • Overview of liquid chromatographic separation methods
  • Application of liquid chromatography
  • Ion chromatographic method
  • Size-exclusion chromatographic method
  • Supercritical fluid chromatography
  • Instrumentation and operating variables
  • Chromatography variables effect
  • Techniques to minimize peak broadening
  • Column condition and regeneration technique
  • Maintenance and installation procedure for inject system, column, pump and detectors
  • Troubleshooting and approaches to solve problems
  • Quantitative methods: calibration methods, external and internal standards, outliers test, determination of analyte concentration, standard addition method, error in quantitative analysis, confidence limits, detection limit, repeatability, reproducibility, and method validation.

Accreditation

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